Uvp Doc-It Life Science Manual do Utilizador descarregar pdf (Página 95)

Performing 1D Analysis

http://marketing.byu.edu/htmlpages/books/pcmds/WARD.html

Neighbor joining: At each stage of clustering the total branch length is minimized. The distance

between two items is approximately the sum of the branch lengths between them. The trees are

not right-aligned and branches can have negative values. Details of the method can be found at

http://www.icp.ucl.ac.be/~opperd/private/neighbor.html

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Web site: uvp.com 1
Table of Contents 2
Introduction 6
Minimum System Requirements 7
On the Fly Activation 8
User Administration 9
Single-User License 9
Usernames and Passwords 10
Technical Support 12
License Agreement 13
Limitation of Liability 14
Navigating the Software 15
Toolbars 16
Image Windows 18
Status Bar 19
Using Plug-In Modules 20
Positioning Plug-in Modules 21
Histogram Controls 22
Digital Video Player 22
Using the Player 23
Player Features 23
Player Options 24
Image Controls Plug-In 26
Change Contrast 27
Change Gamma 27
3D Plots 28
FTP Transfer 29
Log Viewer 29
Zoom/Pan 29
Using Zoom In or Out 30
Magnify Part of an Image 30
Camera Plug-In 32
Capturing Images 35
Creating Templates 36
Creating Colony Templates 37
Editing Images 39
Undo and Redo 40
Using Image Filters 42
Background Filter 43
Burn Changes to a New Image 44
Convert Image 44
Duplicating Images 44
Flip Image 44
Image Enhancement Filters 45
Image History 46
Measurement Tools 47
Merging Two Images 48
Obtaining Image Information 49
Pseudocolor 50
Reduce Image to Mono 51
Resize Image 51
Rotate and Align Image 52
Rulers 53
Scanning Images 53
Spatial Calibration 54
Annotations 56
New Annotation Window 57
Selecting Annotations 58
Formatting Annotations 60
Deleting Annotations 60
Creating Annotations 61
Loading and Saving Images 62
Image File Types 63
The Extended Attributes File 64
Performing 1D Analysis 65
Finding Lanes and Bands 67
1D Analysis Settings 70
Resize Band Extents 71
Delete Lanes and Bands 72
Lane and Band Information 73
Lane Profile Graph 74
Display Multiple Lanes 75
Change the Selected Lane 75
Axis Options 75
Display Options 75
Selecting Background Color 76
Overview 77
Filtering Data 77
Export Data Explorer Reports 80
Using the 1D Analysis Plugin 83
Specific Correction Options 83
Concentration Calibration 84
Unit Type 85
Molecular Weight Calibration 88
Using the Stretch Factor 89
Exact Placement of Bands 90
Adding Rf Lines Manually 91
Generating Dendrogram Graphs 93
Modify Dendrogram Graphs 93
Creating clusters 94
Linkage rules 94
Performing Colony Counting 96
Label Type 97
Colony Marking 97
Label Color 97
Automatic Counting 98
Manual Counting 98
Edit Colonies 100
Add Colonies 100
Delete Colonies 101
Split Colonies 101
Merging Colonies 102
Spiral Counting 102
Zone Analysis 103
Reporting Functions 104
Classes 104
Statistics 105
Distribution 105
Printing Reports and Images 106
Purpose 108
Features 108
Glossary 110

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Uvp Doc-It Life Science Manual do Utilizador Página 95

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